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LncRNA NORAD promoted ferroptosis in <t>ECCs</t> by down-regulating GPX4. ( A ) The Starbase database to analyze the correlation of lncRNA NORAD with GPX4 in EC; ( B ) RT-qPCR to determine the GPX4 level <t>in</t> <t>endometrial</t> tissues, n = 92, GPX4 levels were 1.01 ± 0.10 in paracancerous tissues and 1.44 ± 0.20 in cancerous tissues; ( C ) Pearson’s correlation coefficient to analyze the correlation of lncRNA NORAD with GPX4; ( D ) Western blot to determine GPX4 level of cells; ( E ) CCK-8 to assess cell viability; ( F - I ) Kits to determine cellular GSH, ROS, MDA and Fe 2+ levels; ( J ) LDH kits to determine cellular LDH release; ( K ) Transwell to evaluate cell invasion. The cellular experiments were conducted in triplicate. Data were expressed as mean ± SD. One-way ANOVA was adopted for comparisons among multiple groups, with Tukey’s multiple comparison test employed for post hoc tests. * P < 0.05, ** P < 0.01
Human Eccs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human eccs/product/ATCC
Average 96 stars, based on 1 article reviews
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cell culture medium: dmem/f12 (ecc-1, pc-3)  (Thermo Fisher)
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LncRNA NORAD promoted ferroptosis in <t>ECCs</t> by down-regulating GPX4. ( A ) The Starbase database to analyze the correlation of lncRNA NORAD with GPX4 in EC; ( B ) RT-qPCR to determine the GPX4 level <t>in</t> <t>endometrial</t> tissues, n = 92, GPX4 levels were 1.01 ± 0.10 in paracancerous tissues and 1.44 ± 0.20 in cancerous tissues; ( C ) Pearson’s correlation coefficient to analyze the correlation of lncRNA NORAD with GPX4; ( D ) Western blot to determine GPX4 level of cells; ( E ) CCK-8 to assess cell viability; ( F - I ) Kits to determine cellular GSH, ROS, MDA and Fe 2+ levels; ( J ) LDH kits to determine cellular LDH release; ( K ) Transwell to evaluate cell invasion. The cellular experiments were conducted in triplicate. Data were expressed as mean ± SD. One-way ANOVA was adopted for comparisons among multiple groups, with Tukey’s multiple comparison test employed for post hoc tests. * P < 0.05, ** P < 0.01
Cell Culture Medium: Dmem/F12 (Ecc 1, Pc 3), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell culture medium: dmem/f12 (ecc-1, pc-3)/product/Thermo Fisher
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ecc analysis em-3000  (Tomey Corporation)
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LncRNA NORAD promoted ferroptosis in <t>ECCs</t> by down-regulating GPX4. ( A ) The Starbase database to analyze the correlation of lncRNA NORAD with GPX4 in EC; ( B ) RT-qPCR to determine the GPX4 level <t>in</t> <t>endometrial</t> tissues, n = 92, GPX4 levels were 1.01 ± 0.10 in paracancerous tissues and 1.44 ± 0.20 in cancerous tissues; ( C ) Pearson’s correlation coefficient to analyze the correlation of lncRNA NORAD with GPX4; ( D ) Western blot to determine GPX4 level of cells; ( E ) CCK-8 to assess cell viability; ( F - I ) Kits to determine cellular GSH, ROS, MDA and Fe 2+ levels; ( J ) LDH kits to determine cellular LDH release; ( K ) Transwell to evaluate cell invasion. The cellular experiments were conducted in triplicate. Data were expressed as mean ± SD. One-way ANOVA was adopted for comparisons among multiple groups, with Tukey’s multiple comparison test employed for post hoc tests. * P < 0.05, ** P < 0.01
Ecc Analysis Em 3000, supplied by Tomey Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecc analysis em-3000/product/Tomey Corporation
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ecc  (National Institute of Standards and Technology)
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National Institute of Standards and Technology ecc
LncRNA NORAD promoted ferroptosis in <t>ECCs</t> by down-regulating GPX4. ( A ) The Starbase database to analyze the correlation of lncRNA NORAD with GPX4 in EC; ( B ) RT-qPCR to determine the GPX4 level <t>in</t> <t>endometrial</t> tissues, n = 92, GPX4 levels were 1.01 ± 0.10 in paracancerous tissues and 1.44 ± 0.20 in cancerous tissues; ( C ) Pearson’s correlation coefficient to analyze the correlation of lncRNA NORAD with GPX4; ( D ) Western blot to determine GPX4 level of cells; ( E ) CCK-8 to assess cell viability; ( F - I ) Kits to determine cellular GSH, ROS, MDA and Fe 2+ levels; ( J ) LDH kits to determine cellular LDH release; ( K ) Transwell to evaluate cell invasion. The cellular experiments were conducted in triplicate. Data were expressed as mean ± SD. One-way ANOVA was adopted for comparisons among multiple groups, with Tukey’s multiple comparison test employed for post hoc tests. * P < 0.05, ** P < 0.01
Ecc, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecc/product/National Institute of Standards and Technology
Average 90 stars, based on 1 article reviews
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LncRNA NORAD promoted ferroptosis in ECCs by down-regulating GPX4. ( A ) The Starbase database to analyze the correlation of lncRNA NORAD with GPX4 in EC; ( B ) RT-qPCR to determine the GPX4 level in endometrial tissues, n = 92, GPX4 levels were 1.01 ± 0.10 in paracancerous tissues and 1.44 ± 0.20 in cancerous tissues; ( C ) Pearson’s correlation coefficient to analyze the correlation of lncRNA NORAD with GPX4; ( D ) Western blot to determine GPX4 level of cells; ( E ) CCK-8 to assess cell viability; ( F - I ) Kits to determine cellular GSH, ROS, MDA and Fe 2+ levels; ( J ) LDH kits to determine cellular LDH release; ( K ) Transwell to evaluate cell invasion. The cellular experiments were conducted in triplicate. Data were expressed as mean ± SD. One-way ANOVA was adopted for comparisons among multiple groups, with Tukey’s multiple comparison test employed for post hoc tests. * P < 0.05, ** P < 0.01

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Mechanism of LncRNA NORAD regulating ferroptosis in endometrial cancer cells by modifying GPX4 through FTO-mediated m6A methylation

doi: 10.1007/s00018-025-05855-x

Figure Lengend Snippet: LncRNA NORAD promoted ferroptosis in ECCs by down-regulating GPX4. ( A ) The Starbase database to analyze the correlation of lncRNA NORAD with GPX4 in EC; ( B ) RT-qPCR to determine the GPX4 level in endometrial tissues, n = 92, GPX4 levels were 1.01 ± 0.10 in paracancerous tissues and 1.44 ± 0.20 in cancerous tissues; ( C ) Pearson’s correlation coefficient to analyze the correlation of lncRNA NORAD with GPX4; ( D ) Western blot to determine GPX4 level of cells; ( E ) CCK-8 to assess cell viability; ( F - I ) Kits to determine cellular GSH, ROS, MDA and Fe 2+ levels; ( J ) LDH kits to determine cellular LDH release; ( K ) Transwell to evaluate cell invasion. The cellular experiments were conducted in triplicate. Data were expressed as mean ± SD. One-way ANOVA was adopted for comparisons among multiple groups, with Tukey’s multiple comparison test employed for post hoc tests. * P < 0.05, ** P < 0.01

Article Snippet: Human normal endometrial cells (NECs; SHT290) and human ECCs (SNU-685, Ishikawa, HEC-1B, HEC251), procured from American Type Culture Collection (Manassas, VA, USA), were seeded at a density of 5 × 10 5 cells/well into the dulbecco’s modified eagle medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (Gibco) and then cultured in an incubator at 37 °C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Western Blot, CCK-8 Assay, Comparison